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文献和实验A method to identify p62s UBA domain interacting proteins
The UBA domain is a conserved sequence motif among polyubiquitin binding proteins. For the first time, we demonstrate a systematic, high throughput approach to identification of UBA domain-interacting proteins from a proteome-wide perspective
Testing Rabbit Bleeds By Elisa
same amount of NaBH4 after 15''. Do a quick microfuge spin if there are too many bubbles. B. ELISA with Peptide conjugated to BSA Coat 10 ug/ml antigen (peptide conjugated to BSA) diluted in TBS (50 ul/well) ON at 4 deg C. Remove antigen
Cell death detection in Xenopus embryos by ELISA
(eppendorf) for 10 min. at 4°C Remove 160 µl supernatant / embryo, avoiding pellet contamination of the supernatant Samples can be stored, if not used the same day, in aliquots at -20°C Do ELISA according to manufacturer's instructions.
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