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文献和实验Identification of Scavenger Receptor Ligands
high-throughput screening of complex mixtures. The assay successfully identified bacterial and plasma ligands for macrophage scavenger receptor A and can be adapted to screen for novel exogenous or endogenous ligands for any immune receptor of interest.
Assessing Macrophage Phenotype During Tissue Repair
/macrophage populations can be sorted by magnetic separation. The phenotype of these cells is assessed by real-time PCR, flow cytometry and ELISA. A complementary approach of assessing monocyte/macrophage phenotype by immunofluorescence staining
ELISA-based assay for scatchard analysis of ligand-receptor interactions
plasma membrane of the cells offers the additional possibility of screening factors that interact with the cytoplasmic domain of the receptors for their possible effects on the parameters of the extracellular ligand-receptor interaction.
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