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文献和实验to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
The sandwich ELISA measures the amount of antigen between two layers of antibodies. The antigens to be measured must contain at least two antigenic sites, capable of binding to antibody, since at least two antibodies act in the sandwich
again and washed three times with wash buffer. 9. The color development system is added and the color intensities are measured. 相关仪器试剂设备 酶标仪 洗板机 化学发光检测仪 ELISA检测试剂盒 ELISPOT检测 ELISA试剂盒 抗体 酶标板
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