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文献和实验Preparation of Brain Membrane Fractions
), pH 7.4, 10% sucrose, 5 mM EDTA) containing a freshly added protease inhibitor cocktail of 1 mM phenylmethylsulfonyl fluoride, 20 µg/ml benzamidine, and 20 µg/ml iodoacetamide. For monkey or human brain, remove tissue no later than 4–6 hr postmortem
Preparation of Brain Membrane Fractions for Western Blot Analysis
-acetate (TA), pH 7.4, 10% sucrose, 5 mM EDTA) containing a freshly added protease inhibitor cocktail of 1 mM phenylmethylsulfonyl fluoride, 20 µg/ml benzamidine, and 20 µg/ml iodoacetamide. For monkey or human brain, remove tissue no later than 4�6 hr
DNA WALKING REFERRENCEKi-Bum Park and Suk-Heung Oh(2007)Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3. Bioresource Technology 98(2): 312-319.Young
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