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文献和实验Cell Reports:山东大学马春红团队揭示 Tim-4 在巨噬细胞抗病毒天然免疫和胆固醇稳态调控中的重要作用
巨噬细胞内胆固醇含量,抑制固醇调节元件结合蛋白 2 (Sterol regulatory element binding protein 2,SREBP2) 的活化。接下来,研究人员使用 SREBPs 活化抑制剂 Fatostatin 进行体内、体外病毒感染实验,结果发现抑制 SREBP2 活化后,Tim-4 调控抗病毒应答及病毒复制的作用消失。 未成熟的 SREBP2 与 SREBP 裂解激活蛋白(SREBP cleavage-activating protein,SCAP)组成复合
DETECTION OF RECOMBINANT PROTEINS BY ELISA
Refer to the Western Blot Protocol for preparation of bacterially expressed protein. From a 10 ml bacterial culture, 2-4 plates can be coated for a direct ELISA.Direct ELISA: Lyse and sonicate the bacteria that express the protein
ELISA Detection of Foreign Proteins
Immunoassays were first developed over 30 yr ago. The radioimmunoassay for insulin described by Yalow and Berson (1) heralded a new era in the use of antibody reagents for the quantification of proteins and peptides. The Nobel Prize-winning
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