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文献和实验Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells
Adipose tissue-derived stem cells (ASCs) have been described for a number of laboratory animals and humans. Improved culture conditions and cellular characteristics of ASCs have been identified. ASCs can self-renew and differentiate
Isolation and Cultivation of Porcine Astrocytes
A procedure for the isolation and cultivation of astrocytes from swine is described. More specifically, the donor animals are adolescent minipigs about 3 months in age and 10 kg in weight. About 20 g of cerebral tissue can be isolated
. In a 4oC cold room, carefully and thoroughly remove the meninges and any blood-red tissue from the surface, stem, and within the folds of each brain. Do this by both picking tissue away by hand and wiping with kimwipes. The dry wipe
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