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文献和实验Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes
by the viral gastroenteritis unless they are protected by antibodies. In addition to the cellular receptor, porcine aminopeptidase N, the TGEV spike protein binds to sialic acid residues. We have shown that the sialic acid binding activity mediates the binding
Porcine brain tubulin preparation
the spectrophotometer against the same PM buffer containing 0.5 mM MgGTP) using the extinction coefficient of tubulin = 115,000 Mol/cm ([tubulin] = (A280 x dilution factor)/extinction coefficient)). Add PM buffer containing 0.5 mM MgGTP to adjust the final protein
/cm ([tubulin] = (A280 x dilution factor)/extinction coefficient)). Add PM buffer containing 0.5 mM MgGTP to adjust the final protein concentration to 45 uM. Disburse into several 1 ml aliquots (stock aliquots) and several 50ul aliquots
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