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文献和实验Immunoassays for Lipid Peroxidation End Products: One-Hour ELISA for Protein-Bound Acrolein and HNE
have been prepared (Fig. 1 ) (6,7). However, several difficulties are encountered with enzyme-linked immunosorbent assay (ELISA) determination of haptenic molecules. One is the low avidity of MAbs against the small epitopes in general
gently for 5 min, and check the pH with pH indicator paper. Repeat the mixing settling, aspiration, and filtering of the resin as in step 1. If the pH of the slurry was not above 12, transfer the phosphocellulose to the 4 L beaker, add 2 L of 0.1M
Porcine brain tubulin preparation
, and check the pH with pH indicator paper. 3. Repeat the mixing settling, aspiration, and filtering of the resin as in step 1. If the pH of the slurry was not above 12, transfer the phosphocellulose to the 4 L beaker, add 2 L of 0.1M NaOH, and repeat
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