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文献和实验Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
Culture of Human Smooth Muscle Cells
The wall of a human artery consists of three distinct tunics. The tunica intima is lined by a layer of endothelial cells facing the lumen. Smooth muscle cells (SMCs) are the predominant cell type in the tunica media of arteries
Human Vascular Smooth Muscle Cell Culture
Human vascular smooth muscle cells (VSMCs) in culture are an important tool in understanding how VSMCs function and contribute to vessel wall contraction as well as disease. In this chapter, we describe methodologies that enable
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