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文献和实验Isolation of Actin and Myosin Filaments
7.0 0.001 M EDTA (Ethylene diamine tetra acetic acid) Blender Preparative centrifuge Materials for TEM fixation, embedding and observation Procedure 7 Obtain fresh chicken gizzards 8 and dissect the lateral muscles
. Directions for preparation of the DNaseI column are below in Appendix . Day 1 Extraction of Actin from Chicken Brain (large scale prep from tissue) 1. Homogenize tissue (fresh or frozen) in 1X Extraction Buffer without detergents at a ratio of 100
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
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