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文献和实验Cell death detection in Xenopus embryos by ELISA
(eppendorf) for 10 min. at 4°C Remove 160 µl supernatant / embryo, avoiding pellet contamination of the supernatant Samples can be stored, if not used the same day, in aliquots at -20°C Do ELISA according to manufacturer's instructions.
In Vivo and In Vitro Determination of Cell Death Markers in Neurons
. The goal is to offer an array of assays that, combined, provide both qualitative and quantitative analysis of the relationship between mitochondrial viability and activation of an intracellular cell death process. Immunofluorescence, Western blot, and ELISA
). It has been demonstrated that fgf and shh expression levels are corelated in a manner indicative of a regulatory relationship in the in developmental systems includig the limb (Gilbert 2000). To study the effect of fgf signaling on early chicken development, we interfered
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