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文献和实验In Vitro Selection of Peptides Acting on NMDA Receptors
5 in this volume ( 3 ). Individual isolates from the enriched pools were then characterized by a phage ELISA to identify clones that bound specifically to NR1. The sequence of peptides displayed by phage that bound to receptor was deduced by sequencing
Two long PCR steps: First round of the nested PCR step of the end point dilution procedure to quantitate the cDNA. First round of the nested PCR step of the limiting dilution step to amplify single templates
Long PCR Reagents and Guidelines
George Church Lab, Harvard Medical School PCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main
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