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文献和实验A Solid-Phase Mutual Inhibition Assay with Labeled Antigen
with the respective MAbs, competitor MAbs, biotinylated antigen, and avidin–peroxidase conjugate. Using carcinoembryonic antigen (CEA) as a model antigen, the method was applied to determine epitope specificities of anti-CEA MAbs. A constant amount of biotinylated CEA
on the beads was detected using biotinylated antigen/streptavidin-phycoerythrin. The method was demonstrated by isotyping antibodies directed against an anti-CD11a antibody therapeutic (RAPTIVA� /efalizumab) from the serum of a cynomolgus monkey hyper-immunized
Quantitation of Major Histocompatibility Complex Antigen Endocytosis Using Capture ELISA
Major histocompatibility class I and II (MHC-I and MHC-II) antigens (Ags), as well as a variety of cell surface receptors, have been shown to enter the endocytic pathway of antigen-presenting cells (APCs). MHC Ags endocytose into clathrin
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