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文献和实验Purification of Tubulin from Porcine Brain
-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.
Culture of endometrial explants
Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion of Proteins and Prostaglandins P. J. Hansen and J. G. Betts Dairy Science Dept., University of Florida, Gainesville 32611-0701
to allow the column to pack properly for the next ~48hrs while MT protein is prepared. A well-packed phosphocellulose column should be perfectly even in color with no evidence of "cracks" in the resin. The better the phosphocellulose column is packed
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