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文献和实验Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes
by the viral gastroenteritis unless they are protected by antibodies. In addition to the cellular receptor, porcine aminopeptidase N, the TGEV spike protein binds to sialic acid residues. We have shown that the sialic acid binding activity mediates the binding
Porcine brain tubulin preparation
Homogenization buffer (freshly prepared) 1L PM buffer 100 mM MgATP 1L PMG Buffer 100 mM MgGTP 1M dithiothreitol (DTT) Glutamic acid sodium salt 实验设备 Waring Blender Temperature controlled ultracentrifuge
to allow the column to pack properly for the next ~48hrs while MT protein is prepared. A well-packed phosphocellulose column should be perfectly even in color with no evidence of "cracks" in the resin. The better the phosphocellulose column is packed
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