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文献和实验Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells
Adipose tissue-derived stem cells (ASCs) have been described for a number of laboratory animals and humans. Improved culture conditions and cellular characteristics of ASCs have been identified. ASCs can self-renew and differentiate
Synchronization of In Vitro Maturation in Porcine Oocytes
When removed from the follicles, during the 44 h process of in vitro maturation (IVM) fully grown porcine oocytes resume meiosis spontaneously from the late diplotene stage of the first meiotic prophase and proceed to the metaphase-II (MII
volume, and pair the tubes by weight (to 0.01 g). Discard any extra homogenate that does not fit into a full rotor of centrifuge tubes. Tubulin is sensitive to proteases and easily denatured, and keeping extra homogenate for one hour during the following
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