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文献和实验Synchronization of In Vitro Maturation in Porcine Oocytes
When removed from the follicles, during the 44 h process of in vitro maturation (IVM) fully grown porcine oocytes resume meiosis spontaneously from the late diplotene stage of the first meiotic prophase and proceed to the metaphase-II (MII
/cm ([tubulin] = (A280 x dilution factor)/extinction coefficient)). Add PM buffer containing 0.5 mM MgGTP to adjust the final protein concentration to 45 uM. Disburse into several 1 ml aliquots (stock aliquots) and several 50ul aliquots
Porcine brain tubulin preparation
the spectrophotometer against the same PM buffer containing 0.5 mM MgGTP) using the extinction coefficient of tubulin = 115,000 Mol/cm ([tubulin] = (A280 x dilution factor)/extinction coefficient)). Add PM buffer containing 0.5 mM MgGTP to adjust the final protein
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