- ￥1209
- 华韵生物(Huayunbio)
- HFC2141-20Tests
- 2025年07月14日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
20Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
|
| Alternate Names |
CD11 antigen-like family member A;CD11a;Integrin alpha-L;Itgal;LFA-1A;Ly-15;Lymphocyte antigen 15
|
| Swissprot |
P20701
|
| Gene ID |
3683
|
| Clone No |
TS1/22.1.1.13
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
Elab Fluor® 488 Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
Elab Fluor488
|
| Conjugation Information |
Elab Fluor 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
Alexa Fluor 555 (560121); Alexa Fluor 647 (560122) 1:100 Stringent human pluripotent stem cell marker; Alexa Fluor 647 requires
. PBTB solution: 1x PBS, 0.1% Tween-20, 1% milk powder. 4. Detection of DIG-labeled probe: a. Biotinylated anti-DIG antibody followed by Streptavidin-HRP, recommended to obtain strongest signal: Biotin-conjugated mouse
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
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