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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
20Tests
请联系华韵客服获取说明书。
产品简介:
| Background |
CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-Cells), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.
|
| Alternate Names |
CD5;LEU1;Lymphocyte antigen T1/Leu-1;T-cell surface glycoprotein CD5
|
| Swissprot |
P06127
|
| Gene ID |
921
|
| Clone No |
UCHT2
|
| Host |
Mouse
|
| Reactivity |
Human
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
Elab Fluor® 488 Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
Elab Fluor488
|
| Conjugation Information |
Elab Fluor 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
If the cell surface protein of interest is expressed at high levels, it may be possible to use a mAb that is directly conjugated to Alexa Fluor 488, omitting the need for indirect staining using a biotinylated antibody. If this is the case, omit
Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
Alexa Fluor 555 (560121); Alexa Fluor 647 (560122) 1:100 Stringent human pluripotent stem cell marker; Alexa Fluor 647 requires
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔: Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4412 Anti-Rabbit IgG(H+L), F(ab
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