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- 华韵生物(Huayunbio)
- HFC1865-50Tests
- 2025年07月12日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
50Tests
请联系华韵客服获取说明书。
产品简介：
| Background |
The Cy34.1 monoclonal antibody specifically binds to the B-lymphocyte differentiation antigen CD22 on strains having the Lyb-8.2 alloantigen (e.g., A, BALB/c, CBA, C3H/He, C57BL, C57L, C58, SJL, SWR, but not AKR, DBA/1, DBA/2, NZB, PL). CD22 is expressed at high levels on mature peripheral B lymphocytes (follicular and marginal zone), B-1 cells (CD5+ B cells), and plasma cells. It is a member of the Ig gene superfamily and associates with the B-cell antigen receptor. Its sialic acid- binding immunoglobulin-like lectin (siglec) extracellular region mediates B-cell adhesion to ligands on endothelial cells in the bone marrow. Its intracellular domain is phosphorylated after cross-linking of antigen receptor or MHC class II antigen. It is involved in negative regulation of B-cell activation and protection from autoimmunity. B-cell proliferative responses to LPS or anti-mouse Ig µ chain are augmented in the presence of Cy34.1 mAb.
|
| Alternate Names |
Siglec2;B-cell receptor CD22;B-lymphocyte cell adhesion molecule;BL-CAM;CD22;Cd22;Lyb-8;Sialic acid-binding Ig-like lectin 2;Siglec-2;T-cell surface antigen Leu-14
|
| Swissprot |
P35329
|
| Gene ID |
12483
|
| Clone No |
Cy34.1
|
| Host |
Mouse
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Mouse IgG1, κ
|
| Isotype Control |
PE/Cyanine7 Mouse IgG1, κ Isotype Control[MOPC-21]
|
| Conjugation |
PE/Cyanine 7
|
| Conjugation Information |
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Fluorochrome Absorption and Emission Spectra
. BD Cy-Chrome ™ is a tandem conjugate system, with an absorption maximum of approximately 650 nm (Fig. 2), which combines R-phycoerythrin and a cyanine dye (MW 1.5 kDa). When excited by 488-nm light, the excited fluorochrome (R-PE) is able
Fluorochrome Absorption and Emission
. BD Cy-Chrome ™ is a tandem conjugate system, with an absorption maximum of approximately 650 nm (Fig. 2), which combines R-phycoerythrin and a cyanine dye (MW 1.5 kDa). When excited by 488-nm light, the excited fluorochrome (R-PE) is able
,or write to the author. Identification of Tissue-Specific MicroRNAs from Mouse http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRT-45X0D1F-N&_user=440026&_coverDate=04%2F30%2F2002&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c
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