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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
12个月
- 供应商:
广州华韵生物科技有限公司
- 应用范围:
FCM
- 规格:
25μg
请联系华韵客服获取说明书。
产品简介:
| Clone No |
LTF-2
|
| Host |
Rat
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Conjugation |
None (AF/LE)
|
| Storage Buffer |
0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验Immunohistochemisty-Fluorescence Protocol
Materials Cytokine-specific Primary Antibodies unlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'' or ''BAF'' series) or selected monoclonal antibodies. Secondary Antibodies
200,000 dpm samples in 300 µl IMP buffer. 4) To preclear samples, add 50 µl of control (normal rabbit serum, mouse isotype control, etc.) to each tube and 25 µl of Protein G slurry (shake to suspend slurry before pipetting) and incubate for 1 h at 4 C
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
, add 50 µl of control (normal rabbit serum, mouse isotype control, etc.) to each tube and 25 µl of Protein G slurry (shake to suspend slurry before pipetting) and incubate for 1 h at 4 C. 5) Centrifuge of 1 min on microcentrifuge
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