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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
5 above. Fixation for > 5 minutes can lead to decreased enzyme activity. STAINING FOR ALKALINE PHOSPHATASE ACTIVITY Phosphatase genes are useful reporter genes as several histochemical methods that yield precipitated, highly colored and/or electron
Isolation of Rat Bone Marrow Stem Cells
of hepatocytes for pharmaco-toxicological research and testing. We describe a protocol for isolation of mononuclear cells from adult rat bone marrow.
Generation of Dendritic Cells from Rat Bone Marrow
It has long been known that tissue dendritic cells (DC) are bone-marrow-derived (1 ,2 ). However, early attempts to generate DC from the bone marrow gave only low numbers, presumably because maturation and survival signals required in vivo
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