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文献和实验Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen
for the characterization of these cells, including procedures for testing functional lineage restriction, determination of rearrangement status, analyses of gene expression at the ribonucleic acid (RNA) and protein level, and assessment of cell cycle state.
Generation of Mouse Bone Marrow-Derived Macrophages
Abstract Isolation of resident macrophages from mouse tissues involves complex procedures for a small yield. This is inconvenient for many functional macrophage assays, which require large numbers of relatively homogeneous
Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow
. Different protocols have been developed to differentiate DC from total mouse bone marrow in vitro. Here, we describe the isolation of a specific DC progenitor population, referred to as preimmunocytes, and document protocols for their differentiation
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