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文献和实验RNAi Knockdown of Transcription Factor Pu.1 in the Differentiation of Mouse Embryonic Stem Cells
Murine embryonic stem (mES) cells are pluripotent cells derived from the inner cell mass of the preimplantation blastocyst. These cells are primitive and undifferentiated and have the potential to become a wide variety of specialized cell
on cultured cell lines. More recently, this technique has been adapted to a variety of tissues in different model organisms. We describe here a ChIP protocol on freshly isolated mouse embryonic kidneys for in vivo analysis of transcription factor recruitment
Culturing Mouse Embryonic Fibroblasts
Dissecting tools: Scissors, forceps, scalpel 95% ethanol in a squeeze bottle and in a beaker Mouse breeders Note: This protocol describes the isolation of primary MEFs and passage according to the 3T3 protocol. Passaging primary cells
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