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文献和实验) should be performed for optimization. Add 100l of the cell suspension to each well. For each condition, use triplicate wells. Add soluble antibody in 100l to each well. Titrate antibodies for optimal performance in the assay conditions used. If isolated T
Collection, Separation, and Activation of Human T Lymphocytes
(3 ). In particular, activation of antigen-specific CD4+ T cells of the Th2 subset in the lungs, resulting in interleukin (IL)-5 secretion, plays a major role in allergic inflammation of the airways (4 ). T-cell activation requires two signals, one provided
Techniques for generating human T-cell clones (TCCs) were first described nearly two decades ago (1 , 2 ). This was a direct consequence of the discovery of T-cell growth factor and the subsequent ability to propagate T-cells over extended
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