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文献和实验施一公团队再获新进展!解析人类 pre-tRNA 剪接机制,完善剪接体结构「地图」
长久以来,剪接体的调控机理是怎样的,它们在细胞内部的动态组合和变化是怎样的,深深地吸引着科学家们的研究兴趣,但其神秘的面纱一直未被揭开。 2023 年 4 月 6 日,西湖大学施一公团队在 Molecular Cell 杂志发表研究论文 Structural basis of pre-tRNA intron removal by human tRNA splicing endonuclease,该研究首次解析了人类 tRNA 剪接内切核酸酶(TSEN)在催化前和催化后状态下与全长 tRNA
The Analysis of tRNA Import Into Mammalian Mitochondria
of corresponding genes from another organism (yeast) in the nucleus. We describe how to establish and to analyze such import mechanisms in cultured human cells. In detail, we describe (1) the construction of plasmids expressing importable yeast tRNA derivatives
consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
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