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文献和实验Human Placental Alkaline Phosphatase Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike other AP proteins typically expressed by cells. A few cell types (HeLa cells, for example
Human Alkaline Phosphatase staining of transgenic embryo/tissue
, penetration of stain may be improved by fixing in PBS + 0.2% glutaraldehyde + 0.02% NP-40 + 0.01% NaDC (at 4oC).Rinse in PBS. Heat inactivate endogenous alkaline phosphatase activity by incubation in PBS at 70-75oC for 30 minutes. Rinse in PBS at RT
In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe
The application of in situ hybridization and the underlying rationale are described in Chapter 46. In situ hybridization of nucleic acid probes to cellular targets of biological specimens affixed to a slide has benefited greatly
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