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文献和实验Binding and Inhibition Assays for Siglecs
to those commonly employed in protein-protein interactions, several important aspects have to be taken into account in binding assays with lectin-like receptors such as Siglecs, sialic acid-recognizing immunoglobulin-like lectins. This chapter describes experimental
领域奠基之作:Cell 重磅报道可胞外展示的糖基化修饰 RNA——glycoRNA
人员探究了 sialic acid binding-immunoglobulin lectin-type(Siglec)受体家族是否能结合细胞表面 glycoRNA,使用可溶性 Siglec-FC 试剂,通过流式细胞术检测其与细胞结合。发现 12 种试剂中 9 种可以与 HeLa 细胞结合,其中 Siglec-11 与 Siglec-14 的结合对 RNase A 处理敏感,这些数据支持了细胞表面 glycoRNA 是 Siglec 受体的直接配体。 图片来源:Cell 研究结论
with smooth, acid-and-alkali-resistent, erosion-resistent,and easy-to-clean-and-sterilized steel rods,the cage has a smooth surface,being free from burrs and cracks,and never vulnerable to desealization.Each cage has a feed box and a water-drinker in it.
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