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文献和实验In vitro Reconstitution of Activation of PLC by Ras and Rho GTPases
fusion protein in E. coli. Subsequently, we describe the methodology necessary to reconstitute this protein with K-Ras-4B and RhoA GTPases and measure its activation.
Multiplex Imaging of Rho Family GTPase Activities in Living Cells
. Here, we take as an example a combination of the RhoA biosensor based on a CFP/YFP FRET modality and the Cdc42 biosensor based on organic dyes that change fluorescence as a function of the local solvent polarity. We list the required optical components as well as cellular
Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells
and image analysis. Curr. Protoc. Cell Biol. 46:14.11.1?14.11.26. © 2010 by John Wiley & Sons, Inc. Keywords: biosensors; Rho family GTPases; FRET; live cell imaging
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