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文献和实验Genotyping for Cytochrome P450 Polymorphisms
Protocols for the extraction of DNA from human blood and for genotyping for a number of common cytochrome P450 polymorphisms using either polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR-single-strand
Experimental Approaches for the Study of Cytochrome P450 Gene Regulation
This chapter uses cytochrome P450 1A1 (CYP1A1) as a model “xenobiotic inducible” gene and the aryl hydrocarbon receptor (AHR) as a regulator of this gene product to illustrate how studies may be performed to understand the relationships
1B1 are each active in the oxidation of theophylline (11 ), caffeine (11 , 12 ), estradiol (13 , 14 ), benzo[a]pyrene (11 ) and 7-ethoxyresorufin (11 ). With 7-ethoxyresorufin as substrate, the rank order of specific activity (pmol/min/nmol P450) is CYP1A1
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