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Rabbit CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit实验中的注意事项:1.切记在加酶试剂后用吸水纸在酶标板表面轻拭吸干Rabbit CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit2.ELISA试剂盒洗涤时各孔均需加满液体,防止孔口有游离酶不能洗净。Rabbit CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit3.吸取液体时要选用量程和需要量接近的微量加样器吸,减少误差。Rabbit CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit4.样品稀释液应用加液器加注,并经常校对其准确性。Rabbit CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit5.务必做双孔实验,这样才既能保证数据的准确性,又能反映出试剂盒的精密度。
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发表【中文论文】请标注:由上海酶研生物科技有限公司提供;发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
相关实验
policeman.4. Put the extract into an eppendorf and boil for 5 min., cool on ice.5. Load onto 10% SDS-PAGE. I usually load about 1/5 of the lysate from a 60 mm dish. This is about 100 μg protein for PC12 cells.6. After electrophoresis, electrotransfer
-responsive-element binding protein, in the regulation of neuronal plasticity and memory formation.
mm dish. This is about 100 ug protein for PC12 cells. After electrophoresis, electrotransfer to nitrocellulose membrane. I include in the SDS-PAGE prestained molecular weight markers to check the efficiency of the transfer
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兔 CREB (Cyclic AMP Response Element Binding Protein) ELISAKit
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