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文献和实验Genotyping for Cytochrome P450 Polymorphisms
Protocols for the extraction of DNA from human blood and for genotyping for a number of common cytochrome P450 polymorphisms using either polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR-single-strand
Experimental Approaches for the Study of Cytochrome P450 Gene Regulation
This chapter uses cytochrome P450 1A1 (CYP1A1) as a model “xenobiotic inducible” gene and the aryl hydrocarbon receptor (AHR) as a regulator of this gene product to illustrate how studies may be performed to understand the relationships
The human CYP1 family consists of at least three proteins, CYP1A1, CYP1A2, and CYP1B1 (1 ), CYP1A1 is absent or present at very low levels in human liver (2 , 3 ), but its expression is readily detectable in lung (4 , 5 ). By contrast
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