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- EK-BIO（酶研生物）
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- 2025年07月10日
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
in conjunction with X-Phos as the final electron acceptor for the indoxyl dimerization reaction. When reduced, NBT forms a purple precipitate. Of the cloned phosphatases, the human placental alkaline phosphatase gene, PLAP (Kam et al 1985
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
when choosing a vector for lineage analysis are the reporter gene and the promoter that drives its expression. The reporters that have been used include cytoplasmic lacZ [18], nuclear lacZ [19], human placental alkaline phosphatase (PLAP) [20], and avian gag [21
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