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文献和实验hyaline.Epithelial membrane antigen (EMA)- Monoclonal antibody E29.- Source: Dakopatts.- Dilution 1:10- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cell membrane and/or cytoplasm- A marker for epithelial cells
Screening for P-Glycoprotein (Pgp) Substrates and Inhibitors
P-glycoprotein (P-gp), the product of the human ABCB1 gene and often called MDR1, is the best understood membrane protein known to be involved in the active transport of drugs across biological membranes. In addition to mediating or limiting
In Vitro Characterization of Intestinal Transporter, Breast Cancer Resistance Protein (BCRP)
resistance, and can mediate drug-drug interactions when two drugs, one a substrate and the other an inhibitor or substrate of the same transporter, are co-administered. Thus, the U.S. FDA and the EMA now require that all NCEs be evaluated as substrates
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