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文献和实验of a 50% slurry of Glutathione Sepharose 4B (prepared as described above)to each supernatant and mix gently for 5 min at r.t..Add 100 μl of 1 x PBS,vortex briefly,and centrifuge for 5 sec to sediment the Sepharose beads.Discard the supernatant,repeat
Purification of GST fusion proteins in E.coli GST融合蛋白纯化（方法四）筛选表达
aliquots of the insoluble material for analysis by SDS-PAGE.Transfer the supernatants to fresh tubes,Add 20 μl of a 50% slurry of Glutathione Sepharose 4B (prepared as described above)to each supernatant and mix gently for 5 min at r.t..Add 100 μl of 1 x
.Resuspend beads in 320μl of NETN (~550μl final volume)Aliquot 50μl of beads and add up to 200μl of GST lysate.If Incubate at 4℃ with rocking for 30min.Wash 3x with NETN and add lysate containing protein of interest.Incubate 1-2hr at 4℃ with rocking.Wash 3x
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