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文献和实验Single Cell and Spheroid Collagen Type I Invasion Assay
Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment and requires the infiltration of a dense, cross-linked meshwork of collagen type I extracellular matrix. We use a membrane-free single-cell
in E. coli and their secondary structure was shown by circular dichroism to be identical to wild-type protein, and expression of N-terminally-deleted epsilon 14-3-3 protein showed that the N-terminal 26 amino acids are important for dimerization. Intact
Introduction of human 14-3-3 proteins
and their secondary structure was shown by circular dichroism to be identical to wild-type protein,and expression of N-terminally-deleted epsilon 14-3-3 protein showed that the N-terminal 26 amino acids are important for dimerization.Intact 14-3-3 is a potent
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