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文献和实验引物二聚体带,并且降低引物有效浓度而使PCR 反应不能正常进行[8]。 (8)对引物的修饰一般是在5’端增加酶切位点,应根据下一步实验中要插入PCR 产物的载体的相应序列。 1.1.1 PCR 的引物设计 首先从GenBank 查到VI 型 胶原蛋白 的α1 链的mRMA 序列,序列号为 NM_001848 .其序列长为 4164bp,其中98 到3184 为VI 型胶原蛋白的α1链
Methods for Measuring Type I Collagen Synthesis In Vitro
The excess accumulation of type I collagen within tissues leads to organ dysfunction and occurs as a result of an imbalance between synthesis and degradation. This chapter outlines several methods to assess the in vitro production of type
. Here, we describe the detection of autoAbs to various self-antigens with different tissue restriction patterns which includes collagens, k-α1 tubulin, vimentin, and myosin. We also discuss their relevance in monitoring for rejection following solid organ transplantation.
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