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文献和实验A normal-phase, isocratic high-performance liquid chromatography assay is described for cholesterol 7α-hydroxylation catalyzed by CYP7A1, which corresponds to the first and rate-limiting step in the conversion of cholesterol into bile acids
CYP7A1, which is the only CYP7A subfamily P450 tdentified to date (1 ), catalyzes cholesterol 7α-hydroxylation, the first, and rate-limiting, step in the converston of cholesterol to bile acids. CYP7Al has been Isolated from human liver
9.0 16.07-27.4618.2 7.65-17.6916.5 2-3 豚鼠 1.5 5.5(5.0-6.1) 54.555.3 22.8a1 6.4 a2 18.9 8.18.0 14.611
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