猴 CA3 (Carbonic Anhydrase III,

非人灵长类实验动物饲养管理的操作规程二

，cannot be added without official permission． 饲料配方要充分考虑动物的生理习性，并注意饲料的适口性，如猴为素食行动物，喜食水果、蔬菜及玉米等粮食作物。饲养中要注意合理搭配。为保证其营养需要，成猴粗蛋白含量一般应达到16%，幼猴还应高些，应达18～20%。应注意钙磷比例，补充维生素C。为达到饲养标准，满足动物粗蛋白质及代谢能的基本需要，其饲料配方应由有专长的人根据饲料品种、季节变化、饲料来源制定相应配方，并定期检测、测定饲料营养成份。 The feeds

dissociated neurons for patch clamp or Immunohistochemistry

         Bunsen burner              50 mL centrifuge tubes (should be sterile) 35-mm plastic Petri dish with lid, preferably coated             1)       Prepare hippocampal brain slices from rat.   To obtain specific isolated

Method for Single-Cell Electroporation

body regions of the optic tectum of the Xenopus tadpole brain, and of the CA1 and CA3 regions of the rat hippocampal slice) made it likely that the electrode tip would be in close contact with a cell somata. In preparations with less dense cell bodies