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文献和实验5. (take residue) extract at +4 oC with H2O (2x) and filter 6. (take residue) extract at +20-25 oC with acetone (5x) and filter 7. (use muscle acetone powder) extract with buffer G at 0 oC; 20 ml/7 gram acetone powder; centrifuge at 35,000 x g for 30 min
(and any other paraphernalia that came into contact with the sample) to remove all traces of sample and proceed to the protein isolation of the next tissue sample. 9. Spin samples at top speed in the microfuge (4oC for 15+ minutes). 10. Transfer the liquid supernatant
Preparation of protein extracts for western blot 1.Grow cells to mid-log (OD600 less or equal to 1.0). 2.Harvest about 5 OD's of cells in a 13X100 glass dispo tube (Use new tubes.Can be up to about 6 mls of culture). 3.Pellet 3-5 min.in Tomy,3K
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