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文献和实验Isolation of High-Molecular-Weight DNA from Animal Cells
such methods for all preparations of DNA from mammalian cells. The first three methods described below are derived from that of Blin and Stafford (5 ) and should yield high-mol-wt (HMW) DNA from solid tissues, blood, or cells in culture suitable
. Due to their stability and relative simplicity BAC libraries are most preferred over other approaches for cloning large genomic DNA fragments for large-insert libraries. Isolation of intact high molecular weight (HMW) DNA is a critical step underlying the success
【摘要】 目的 了解饲喂金霉素后药物在猪体内的残留情况。方法 应用高效液相色谱仪测定猪部分组织中金霉素的含量,根据残留量,确定临床应用金霉素的休药期。结果 HPLC法稳定性好,测量精度高,最高回收率达87.8%,最低回收率达53.6%。结论 连续5天以600ppm剂量饲喂猪的安全休药期为27天。 【关键词】 金霉素;高效液相色谱;猪;残留 HPLC detection of residual in swine afcer fed
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