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文献和实验Protein Expression and Purification of RPA70-AB (181-422)
Comments: pSV281 RPA70AB Constructed by L.Mizoue; May '02.This plasmid encodes RPA 70 subunit residues 181-422 as a fusion protein with an NT 6His tag.The tag can be cleaved off with TEV protease (recognition site is ENLFYQG)leaving "GS" at NT
Hsp70: Anti-apoptotic and Tumorigenic Protein
Heat shock protein 70 (Hsp70) is a powerful chaperone whose expression is induced in response to a wide variety of physiological and environmental insults, including anticancer chemotherapy, thus allowing the cell to survive to lethal
70% Ethanol Fixation for Recovery of DNA, RNA, and Protein This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following
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