大鼠 MAPτ (Microtubule Associate

A Sensitive and Continuous Fluorometric Activity Assay Using a Natural Substrate: Microtubule-Associated Protein 2

-associated protein 2 [MAP2] (2 ). The assay depends upon the observation that release of small peptides carrying only one fluorescent label, by digestion of a protein labeled with many fluorescent groups, is accompanied by a significant increase

微管结合蛋白(microtubule-associated proteins, MAPs)

-X,作为同带负电的微管蛋白结合的位点。这些位点可中和微管中微管蛋白间的电荷, 维持聚合体的稳定。 Ⅱ型MAP包括MAP2、MAP4、Tau。这些蛋白有几个与微管蛋白结合的18氨基酸重复序列。 MAPs具有多方面的功能∶①使微管相互交联形成束状结构，也可以使微管同其它细胞结构交联。②通过与微管成核点的作用促进微管的聚合。③在细胞内沿微管转运囊泡和颗粒，因为一些分子马达能够同微管结合转运细胞的物质。④提高微管的稳定性∶由于MAPs同微管壁的结合，自然就改变了微管组装和解

Tau Phosphorylation Both In Vitro and in Cells

Tau was originally isolated from brain microtubules and shown to be a microtubule-associated protein (MAP) that promoted tubulin polymerization (1 ). It is largely confined to axons, where it is the major MAP. It promotes microtubule nucleation