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文献和实验A normal-phase, isocratic high-performance liquid chromatography assay is described for cholesterol 7α-hydroxylation catalyzed by CYP7A1, which corresponds to the first and rate-limiting step in the conversion of cholesterol into bile acids
for most of the cholesterol 7a-hydroxylase acttvtty in human liver microsomes (4 ). Thus, mlcrosomal cholesterol 7α-hydroxylase activity can be used as a marker for human liver CYP7A1. Several analytical methods have been developed to quantify hepatrc mrcrosomal cholesterol
性高血压病人常伴随糖、胰岛素和脂蛋白代谢异常,而继发性高血压病人中无此现象。现有的研究资料仅对IR或糖尿病动物模型报道较多,但有关典型X综合症动物模型报道甚少且成功率和重复率低。为此,我们经反复探索,找到了一种成功率高、重复性好的造模方法:即利用两肾一夹(two-kidney,one-clipped:2K1C)加高果糖喂养SD大鼠建立X综合症动物模型,为X综合症的实验研究提供了一种理想的动物模型。1、材料和方法1.1 实验动物和分组 清洁级雄性SD大鼠20只,体重(200±20)g。大鼠随机分成两组:对照
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