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文献和实验-1. In addition to the forkhead and NF-KappaB families, E2F-mediated transcription can also be activated by the hyperphosphorylation and subsequent inactivation of Rb (Retinoblastoma Protein) in response to signals from PI3K and its downstream effectors, PKB
Introduction of human 14-3-3 proteins
inhibitor of protein kinase C,but the N-terminal domain does not inhibit PKC activity.Site-specific mutagenesis of several regions in the tau isoform of 14-3-3,including the mutation of a putative pseudosubstrate site to a potential substrate sequence
) PROTOCOL FOR YEAST PDF - http://research.stowers-institute.org/jeg/2004/cohesin/protocols/ChIP.pdf Protocol derived from a paper by Miriam Braunstein and is based on work from the Allis lab. Chromatin Immunoprecipitation (ChIPs) Cloning Protocol
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