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文献和实验Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization
Despite the identification of numerous deubiquitinating enzymes (DUBs) in recent years, the large majority of this class of enzymes has not been well characterized. This chapter describes biochemical methods that can be used to characterize
for preparing assay reagents, performing HTS for USP14 inhibitors, and carrying out post?HTS analysis. Curr. Protoc. Chem. Biol. 4:311?330 © 2012 by John Wiley & Sons, Inc. Keywords: 26S proteasome; USP14; deubiquitinating enzyme; high
A Method for Assaying Deubiquitinating Enzymes
for ribosome biogenesis as well as of free Ubs. Deubiquitinating enzymes (DUBs) are known to consist of a large protein family in eukaryotes. For example, the budding yeast has 17 genes for DUBs (4 ). Moreover, so far more than 60 full-length DUB sequences
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