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文献和实验TSA Treatment of Mammalian Cells
) towards the nuclear periphery. HP1 domain disruption upon TSA treatment is monitored by immunofluorescence using an antibody against HP1alpha (2HP1H5) from EUROMEDEX. Reversibility of the drug effect was obtained within 20 hours after TSA removal. (Figure
TSA Treatment of Mammalian Cells (PROT09)
heterochromatin domains) towards the nuclear periphery. HP1 domain disruption upon TSA treatment is monitored by immunofluorescence using an antibody against HP1alpha (2HP1H5) from EUROMEDEX. Reversibility of the drug effect was obtained within 20 hours
detection involves enhancement of fluorescent signals by tyramide signal amplification (TSA) and effective inactivation of the antibody–POD conjugate prior to the following detection round. We provide a detailed description of embryo preparation
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