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文献和实验Ribonuclease inhibitor (RI) is a cytoplasmic protein of ∼50 kDa that tightly binds and inhibits ribonucleases (RNases) from the pancreatic superfamily (1 ). Diverse RNases with very limited sequence similarities, including RNase
The traditional method for combating RNases in enzymatic reactions such as in vitro transcription, reverse transcription and translation is to use human placental ribonuclease inhibitor (also known as RNase Inhibitor Protein, RI or hPRI
Scale-Up of Single CellInoculated Suspension Cultures of Human Embryonic Stem Cells
by serially upscaling 2-ml static cultures to 50-ml spinner flasks. Use of the Rho kinase (ROCK) inhibitor Y-27632 (Ri) was found to enable cell survival in suspension by promoting hESC reaggregation. This treatment resulted in 44% (vs. 5–10% in untreated
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