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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
40 1 Rail carrier for X26, square 40mm length Newport CN26-40 4
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
, dilute 1000x Me-JA 98% solution, 5ul/1ml EtOH, 100ul/9.9ml Inc. Firmenich Geneva buffer/media, dile 10 or 100x. Protein synthesis inhibitors cycloheximide: [active] 20mM, stock 10mM, MW 281, stock = Boe 103675 2.8mg/ml EtOH, dilute 1000x
- or PAGE or agarose gel electrophoresis of nucleic acids - of known molecular weight along with the protein or nucleic acid to be characterised. A linear relationship exists between the logarithm of the molecular weight of an SDS-denatured polypeptide
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