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文献和实验Measurement of Phosphoinositide 3-Kinase Activity
The term “PI 3-kinase” is now applied to a growing family of proteins that are able to convert phosphatidylinositol (PtdIns), PtdIns(4)P and PtdIns(4,5)P 2 into PtdIns(3)P, PtdIns(3,4)P 2 and PtdIns(3,4,5)P 3
Measurement of Phosphoinositide 3-Kinase and Its Products to Study Adipogenic Signal Transduction
depends on the activation of phosphoinositide 3-kinase (PI3K). This chapter describes the methodology used to measure the cellular accumulation of phosphoinositide products of PI3K. This approach involves labeling the cells with myo-[2—3 H]-inositol
Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC
The phosphoinositide 3-kinase (PI3K) family catalyses the addition of a phosphate group to the D-3 position of polyphosphoinositides (PPIn). Since the discovery in the late 80s that phosphatidylinositol is phosphorylated in the D-3 position
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